In Vitro Exposure to Bisphenol A, Benzophenones 2 or 3, Have Dierent Eects on GnRH Gene Expression and Secretion in Mature and Immature GnRH Neurons

CAMILA MARIGLIANO; CARLOS LIBERTUN; PAULA AROCENA; VICTORIA LUX-LANTOS; JUAN MANUEL RIAÑO GÓMEZ; MARINA FERNANDEZ

New Orleans

Congreso; ENDO2019, 101st Annual Meeting of the Endocrine Society; 2019

The Endocrine Society

Bisphenol A, (BPA), a component of polycarbonate plastics, and Benzophenones (BPs), UV filters, are endocrine disrupting chemicals (EDC). Previously we showed that the in vitro exposure to BPA decreased Kiss-induced GnRH expression in GN11 cells (Dr. Susan Wray, NIH), immature GnRH neurons (1). In this study, we analyzed the in vitro effects of BPA, BP2 and BP3 (1x10-9M), or medium as control (C) in mature GnRH neurons (GT1-7 cells, donated by Dr Pamela Mellon, UCSD, USA, and isolated hypothalami from adult Balb/c males). Effects of BP2 and BP3 (1x10-9M) were also analyzed in GN11 cells. We exposed the cell lines for 24 h and the hypothalami for 6 h to the EDC. GT1-7 and GN11 cells were further incubated with Kiss (1x10-9M, Phoenix Pharmaceuticals, Inc, CA, USA) for 4 h to analyze gene expression (qPCR) and for 1 h to analyze GnRH secretion (RIA). Hypothalami exposed to BPA showed increased GnRH expression compared to C (Relative expression: C:1.0±0.2; BPA:2.2±0.5; Student T-test: BPA vs C p