Phototherapy in female reproductive medicine: effects on ovarian function and possible uses in oncofertility

N PASCUALI; FERNANDA PARBORELL; OUBIÑA G,; ABRAMOVICH DAHIA; SCOTTI L,

Concon

Congreso; XXIX Reunión Anual de la Sociedad Chile; 2018

It is known that LLLT has beneficial effects on several pathological conditions including wound healing, pain and inflammation. LLLT modulates biological processes, including cell proliferation, apoptosis and angiogenesis. In the ovary, these processes are intimately related in terms of fertility. Premature Ovarian Failure (POF) is characterized by the disappearance of ovarian follicles in young women, which may be caused by chemotherapy. POF treatments, which consist mainly of hormone therapies, are not completely effective. The present study proposes photobiomodulation as a strategy to protect the ovary in cancer patients undergoing chemotherapy. The objectives were: to evaluate the in vivo effect of LLLT on ovarian function a) in adult mice model, and b) in POF model induced by chemotherapy. For objective a, F1 mice (BalbC x C57 / BL6) (8 weeks) were used and LLLT (100 and 200 J/cm2) was applied. For the purpose b), POF model was induced with cyclophosphamide (CTX, 75mg/weight) in F1 mice and LLLT (200 J/cm2) was applied. a) Adult mice model: LLLT (200 J/cm2) increased the % of primary and preantral follicles, whilst decreasing the percentage of corpora lutea compared to control ovaries (p