First evidence of physiological alterations in recently develop KISS1 GABA1KO mice.

NOELIA DI GIORGIO; BERNHARD BETTLER; FLORENCIA N TABARES; CARLOS LIBERTUN; MARIANNE BIZZOZZERO HIRIART; VICTORIA LUX LANTOS; NADIA S BOURGUIGNON

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias. 13 a 17 de noviembre 2017.; 2017

Sociedades Argentinas de Biociencias

We have shown that global GABAB1KO mice have alterations in the reproductive axis, especially females, which showed disrupted estrous cycles and reproduction impairment. Moreover, GABAB1KO mice showed coexpression of GABAB receptors (GABABRs) in Kiss1 neurons and a dramatic increase in Kiss1 expression in extrahypothalamic areas (amygdala, BNST) while their expression did not differ from WTs in AVPV-PeN or ARC. Recent results show constant (morning and afternoon), high kisspeptin neuron activation in a proestrous-like environment and failure of ovulation in these mice. To establish the impact of GABABRs on Kiss1 neurons our aim was to develop a strain of mice with specific deletion of GABABRs in Kiss1 cells/neurons and characterize them from a reproductive and metabolic perspective. Kiss-Cre mice (Jackson?s Lab) were crossed with GABAB-floxed mice (donated by Dr. Bettler) to obtain Kiss1-GABAB1KO (KO) mice. Body weight (BW) was evaluated from postnatal day (PND) 7 to PND84. Sexual differentiation was evaluated by ano-genital distance (AGD) on PND7, PND14 and PND21 and relativized to body weight. KO females had increased BW vs WTs [BW (g) PND84: F-KO=29.6±1.2 (n=5) vs F-WT=25.3±1.4 (n=7), p