The hyaluronan synthesis inhibitor 4-methylumbelliferone (4mu) effect on endometriosis (EDT).

OLIVARES C.; ALANIZ LD; STELLA I.; BILOTAS M.; RICCI A.; MADANES D; FERELLA L; BARAÑAO RI; MERESMAN GF

C.A.B.A.

Congreso; REUNION CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

SAB-SAIC-SAIB-SAI-SAA-SAH-SAP-SAB-SAFE-SAFIS

It is known from previous studies that 4MU has antiproliferative and antiangiogenic properties. We evaluated the effect of 4MU in EDT models. Stromal T-HESC and epithelial ECC-1 endometrial cell lines, were treated with different doses of 4MU (ranging 0-4mM) and cell proliferation (CP) by the WST-1 assay was evaluated. Mice were surgically induced with EDT and treated orally with 4MU during 28 days. Six groups were included in the in vivo experiment, two different time points for initiating the treatment (2 and 15 days) and two different doses of 4MU (80 and 200mg/kg) with the appropriate vehicle control groups. The number and size of developed lesions were evaluated, as well as vascularization, CP, infiltrating lymphocytes and collagen depot within the endometriotic lesions. CP of T-HESC was significantly inhibited with 2 and 4mM 4MU (p