IDENTIFICATION OF MOLECULAR MARKERS IN BLADDER CANCER USING MURINE AND HUMAN MODELS

LAPYCKYJ, LARA; GIUSTINA, SILVINA; LODILLINSKY, CATALINA; TEJERIZO, JC; GONZALEZ, MI; EIJÁN, ANA MARÍA; VAZQUEZ-LEVIN, MÓNICA HEBE

Buenos Aires

Congreso; XII Jornadas Científicas de la Sociedad Argentina de Biología; 2010

Sociedad Argentina de Biología

Introduction: Bladder cancer affects over 1 million people worldwide, but the molecular bases remain unknown. Downregulation or loss of epithelial cadherin (cadE) is a prognostic marker for this cancer. The presence of negative modulators of its expression (Twist, Snail) and of mesenchymal cadherins (Neural cadherin, cadN) has been associated to tumor progression. Dysadherin (Dys) is a transmembrane glycoprotein that regulates cadE adhesions functions. Objective: To evaluate the expression of cadE and its modulators in 3 bladder cancer models: a) an orthotopic animal model (MB49/MB49-I), b) a human invasive cell line, T24, c) human tumors Methods: RT-PCR, Western Immunoblotting and immunocitochemistry. Results: In the 3 models we found that cadE expression was downregulated and cadN and Snail were upregulated. For the first time we describe the expression of Dys in bladder tumors of human and murine origin. Discussion: This study describes the identification of Dysadherin, a novel molecular marker in bladder cancer progression