The Cuprizone model under neurosphere´s scrutiny

SILVESTROFF L; FRANCO P; MOLINARI Y

Córdoba

Congreso; XXXIII Reunión Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); 2018

American Society for Neurochemistry

Cuprizone (CPZ) is a copper-chelating agent which induces demyelination in mice. Although its neurotoxic mechanism is still unknown, CPZ has been shown to produce astrogliosis, microglial activation and loss of oligodendrocytes throughout the brain resulting in demyelination and neurotoxicity. Neural stem and progenitor cells (NSC/NPC) are able to generate all neural cell types and can be cultured as neurospheres (NS). NS can be maintained in a proliferative and undifferentiated state or alternatively be forced to differentiate into neurons, astrocytes or oligodendrocytes. In the present work we used NS cultures to evaluate CPZ effects on NSC/NPC survival, proliferation, migration and differentiation.