Contribution to the knowledge of the parotid saliva proteome

T. FERRARY; S. DEL CANTO; D. OLSEN; C. PAVAN; M. BISCOGLIO

FOZ IGUAZU

Congreso; 90th General Session & Exhibition of the IADR - IADR Latin American Regional Meeting; 2011

International Association of Dental Research

Objective: The saliva proteome includes a number of proteins involved in the homeostasis of the oral cavity and there is currently great interest in its utilization as a diagnosis tool. Our objective was to contribution to the knowledge of the saliva proteome and its importance as a diagnosis tool Method: In this work we studied parotid saliva samples from normal children as well as those from children with JIA (Juvenile Idiopathic Arthritis). The 2D-polyacrylamide gel electrophoresis denoted their high complexity degree. Then, two methodologies were used: a) samples were resolved by molecular filtration and fractions analyzed by HPLC-ESI-MS; b) samples were digested by trypsin and then analyzed by nano HPLC-ESI-MS/MS Result: Results indicate sample degradation prior to saliva analysis and many peptides from parotid saliva proteins, such as alpha amylase and proline rich proteins were found. On the other hand, several proteins were described in normal human saliva for the first time (an actin-binding protein, an alpha enolase, fragments of the immunoglobulin kappa chain, a thioltransferase I, a leucocyte lelastase inhibitor, apoliprotein A1). In addition, we found other two proteins only present in JIA samples and never described in normal human saliva. We also reported the finding of a 18460 Da molecular mass present in all JIA samples but absent in normal ones Conclusion: One or both of them could also be potential markers of this pathology and must be submitted to a validation process, which is outside the scope of this work.