Acetaminophen induces oligodendrocyte precursor cell death and reactive astroglia in a primary mixed glial cell culture

PÉREZ MARÍA JULIA; TALLIS SILVINA; ANALIA NOVAK; AMALIA DELFANTE; MODESTO CARLOS RUBIO; CAROLINA INÉS GHANEM

San Diego, CA

Congreso; Experimental Biology 2012. American Society for Pharmacology and Experimental Therapeutics (ASPET); 2012

American Society for Pharmacology and Experimental Therapeutics (ASPET)

Brain damage in acetaminophen (AP) overdose is associated with liver failure but its direct effects has not been evaluated. Aim: To study the effect of toxic and non-toxic doses of AP on astrocyte and oligodendrocyte precursor cell (OPC) viability in a primary mixed glial cell culture. MYM: Primary cultures of mixed glial cells were carried out according to McCarthy and de Vellis (1980). Cells were treated with vehicle (DMSO) or AP (1 and 20 mM). After 48 hs cell viability was assessed; astrocyte marker (GFAP) and OPC marker (PDGFR-alpha) expression was evaluated by western-blot (WB) and immunocytochemistry. Results: The 1 mM dose of AP did not produce significant differences in cell viability, while 20 mM exposure decreased in 40% cell viability (P