GRAPE POMACE EXTRACT (GPE), RICH IN POLYPHENOLS, ENHANCE IRISIN AND PROMOTES ADIPOSE TISSUE BROWNING IN HFD-RATS.

RODRIGUEZ LANZI C; PERDICARO DJ; FONTANA A; OTEIZA P; VAZQUEZ PRIETO MA

Simposio; First International Symposium of Food, Nutrition, Physical Fitness and Health; 2019

Increased visceral adiposity plays an important role in the physiopathology of obesity and is related to altered glucose tolerance. Thus, promoting strategies to prevent these complications might be relevant. Concerning to this, phenolic compounds (PC) are molecules widely distributed in fruits and vegetables with proved beneficial effects. In addition, grape pomace extract (GPE) is a concentrate product obtained from a winemaking-residue with a relative high concentration of PC, (-)-epicatechin (EC) is one of the most relevant. On the other hand, Irisin is a novel myokine activates by PGC-1α in exercising muscles, that is released into the bloodstream after cleavage of fibronectin type 3 domain containing protein 5 (FNDC5). Irisin is related with adipose tissue ?browning? and increased glucose tolerance. Recent evidence suggest that PC are able to activate Irisin. Objetive: The aim of this study was to evaluated the effects of GPE supplementation: i. on epididymal adipose tissue (eWAT) ?browning? and glucose tolerance in a HFD model and ii. in the activation, secretion and possible underling mechanism of FNDC5/Irisin pathway in a HFD model and L6 myotubes. Results: GPE prevents HFD-induced adipocyte hypertrophy and insulin resistance. In adittion, GPE activates eWAT ?browning? evidence by increased protein levels of PGC-1α, PPARγ, PRDM16 and UCP-1. Finally, GPE activates FNDC5/Irisin pathway and enhances Irisin secretion in vivo and in vitro, at least in part via PGC-1α activation. Conclusions: Together, our findings demonstrate that GPE promotes eWAT ?browning?, which is associated with a FNDC5/Irisin pathway activation. This can in part underlie the GPE capacity to prevent HFD-induced adiposity and insulin resistance.gra