IQUIFIB   02644
INSTITUTO DE QUIMICA Y FISICOQUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
?Improvement of soybean nodulation by bacterial exposure to nanoparticles?
Autor/es:
GROPPA M.D.; DE VALOIS N.; ZAWOZNIK M.S.; BENAVIDES M.P.; IANNONE M.F.
Reunión:
Jornada; XX Jornadas anuales de la Sociedad Argentina de Biología (SAB)- XVII Jornadas de la Sociedad Uruguaya de Biociencias (SUB), Segundas Jornadas Rioplatenses de Biología Nanobiotecnología: Pequeñas soluciones para grandes problemas; 2018
Resumen:
Área temática: Biología Animal y Vegetal o - Biotecnología Animal y VegetalIS Improvement of soybean nodulation by bacterial exposure to nanoparticles. De Valois N (1), Zawoznik M S (1), Groppa M D (1,2), Benavides M P (1,2), Iannone M F (1,2)*(1)Cátedra de Química Biológica Vegetal, Facultad de Farmacia y Bioquímica, UBA, CABA, Argentina. (2)IQUIFIB (UBA-CONICET), Facultad de Farmacia y Bioquímica, UBA. CABA, Argentina. *Email: mflorenciaiannone@gmail.com In the last years, the impact of the use of nanotechnology products in the environment was a matter of study. Since Rhizobium-legume symbiosis is a relevant system in agriculture, the goal of this work was to carry out basic studies concerning the effects of magnetite nanoparticles (NPs) on soybean-Bradyrhizobium japonicum symbiotic association. The treatments used were: soybean seeds inoculated with B. japonicum (Control: C) or B japonicum exposed to 10 ppm of magnetite NPs (BANP). B japonicum used for inoculation was previously grown for 5 days in a rotary shaker with or without NPs. For inoculation XXX UFC were used. Seeds were left in contact with the bacterial suspension (C or BANP) for 12 hours. Soybean plants were grown on soil, with periodic water irrigation in a culture chamber and harvested after 20 or 30 days of growth. NPs improved the germination rate of soybean seeds. Additionally, root and aerial part length and total biomass were significantly greater in BANP plants. In the same way, root surface increased four times and an increment of 13% in chlorophyll content was observed in BANP treatments with respect to controls at any time studied. At 20 days, nodules were only present in BANP treatments while leghemoglobin content of BANP plants doubled the content of the controls at 30 days of growth. We concluded that incubation of bacteria with magnetite NP stimulated seedling growth and chlorophyll biosynthesis, as well as favorably impacted on nodulation and biological nitrogen fixation. Thus, magnetite NP could be a good candidate for the design of new products for agricultural use.