Grape pomace extract reduced reperfusion arrhythmias in rats with a high-fat-fructose diet.

PERDICARO DJ; RODRIGUEZ LANZI C; ANTONIOLLI A; FONTANA A; BOTTINI R; MIATELLO RM; DIEZ ER; VAZQUEZ PRIETO MA

Congreso; XXIV Congreso Argentino de Hipertensión Arterial. Interamerican Society of Hypertension; 2017

GRAPE POMACE REDUCED REPERFUSION ARRHYTHMIAS IN RATS WITH A HIGH-FAT-FRUCTOSE DIETPerdicaro DJ1, Rodriguez Lanzi C1, Antoniolli A3, Fontana A3, Bottini R3, Miatello RM1, Diez ER2, Vazquez Prieto MA1.1Laboratory of Cardiovascular Pathophysiology and 2Electrophysiology, UNCuyo and Institute of Medicine and Experimental Biology of Cuyo (IMBECU)-CONICET, and 3Laboratory of Plant Biochemistry, IBAM, FCA, CONICET-UNCuyo. Mendoza, Argentina.Metabolic syndrome (MetS) is a risk factor of sudden cardiac death. Grape pomace (GP), obtained from winemaking process, contains phenolic compounds with potential cardioprotective effects. The aim of this study was to evaluate if a high-fructose-fat (HFF) diet facilitates the occurrence of arrhythmias during the reperfusion, and if a GP supplementation could counteracts this effects. Eight weeks male Wistar rats were randomly assigned for 6-week to the following 3 groups (8 rats per group): Control diet (C), High Fat-Fructose diet (HFF) (20 % bovine fat and 20 % fructose w/w added to the standard chow), and HFF supplemented with Grape pomace (HFF+GP) in a dose of 1g Kg-1day-1. HFF diet induces characteristic features of MetS (higher systolic blood pleasure, dyslipidemia and insulin resistance) which were attenuated by GP supplementation. In addition, HFF induced increased heart weight and reperfusion arrhythmias that were reduced upon GP supplementation. Moreover, GP improves heart p-AKT and p-eNOS expression and reduces Nox4 expression enhanced by a HFF diet, indicating that GP may increase NO? bioavailability in heart. GP counteracts diet-induced heart dysfunction and metabolic alterations. The potential of GP as source of natural bioactive compounds could revalorize this byproduct of winemaking process.