Maternal conjugated linoleic acid modulates triacylglyceride metabolism in adult offspring rats

BERNAL, CLAUDIO; LAVANDERA, JIMENA; GERSTNER, CAROLINA; SAIN, JULIANA; FARIñA, ANA; GONZALEZ, MARCELA; VERA CANDIOTI, LUCIANA; MANCINI SONIA; WILLINER, MARíA ROSA

CABA

Congreso; 21st INTERNATIONAL CONGRESS OF NUTRITION; 2017

World food programme

Conjugated linoleic acid(CLA) might regulate the lipid liver and adipose tissue accretion. Since thereis an association between maternal nutrition, fat depots and risk of offspringchronic disease, the aim was investigate the effect of maternal CLA consumptionon triacylglycerol (TAG) regulation and someinflammatory parameters in adult male rat offspring receiving or not CLA. Female Wistar rats were fedcontrol (C) or CLA-supplemented (1%,w/w) diets during 4 weeks before and throughoutpregnancy and lactation. After weaning, male offspring of CLA rats were fed Cor CLA diets (CLA/C and CLA/CLA groups, respectively), while C male offspringrats were fed C diet (C/C group) for 9 weeks. SerumTAG concentration, liver TAG content, lipogenic and oxidativeenzyme activities, gene expressions, transcriptional factors (SREBP1c, PPARáand PPARã) and inflamatory cytokines mRNA levels; and epididymalwhite adipose tissue (EWAT) LPL activity were measured. Serum TAG levels were increasedin CLA/CLA (+65%) and CLA/C (+92%) groups, associated with a reduction of EWATLPL activity (-41%) and (-67%), respectively. Liver TAG levels were decreased in CLA/CLA (-22%)group relatedto a significant reduction of FAS (-37%) and ACC (-27%) enzyme activities, aswell as of FAS (-44%), ACC (-48%), SCD1 (-54%) and SREBP1c (-25%) mRNA levels,without changes in â-oxidation parameters. Even though in CLA/C rats anormal liver TAG levels were founded, FAS (-38%) and ACC (-31%)activities, as well as ACC (-62%) expression were significantly diminished,associated with a lower expression of the key enzyme of â-oxidation (CPT-Ia)(-23%), PPAR-á (-22%) and PPAR-ã (-45%). Liver IL-1â gene expression showed asignificant decrease in both, CLA/CLA and CLA/C groups. No changes were observed inIL-6 mRNA levels. These results demonstrate that maternal CLA supplementation programs malerat offspring liver lipid metabolism leading to a prevention of the TAGaccretion in adipose tissue and liver. The present findings could be importantto develop some dietary strategies to reduce the incidence of obesity and fattyacid liver disease in humans.