Effect of a functional milk fat enriched in rumenic acid and vaccenic acid on lipid and carbohydrate metabolism in rats fed a high fat diet

BERNAL, CLAUDIO; SAIN, JULIANA; GERSTNER, CAROLINA; LAVANDERA, JIMENA; SCANAROTTI, IGNACIO; GONZALEZ, MARCELA; ANTONACCI, LILIANA; GAGLIOSTRO, GERARDO

CABA

Congreso; 21st INTERNATIONAL CONGRESS OF NUTRITION (IUNS); 2017

World food programme-Sociedad Argentina de Nutrición

Background and objectives: Intake of high-fat (HF) diets has been associated with steatosis, hepatic lipid alterations and insulin resistance in humans and in experimental animal models. Since rumenic acid (RA) and vaccenic acid (VA), naturally present on dairy products, showed to induce health bene!ts on lipidand carbohydrate metabolism, some e"orts have been made to increase their levels in the milk fat (MF) and obtain a functionalmilk fat (FMF). #e aim of the present study is to investigate parameters related to glucose and triacylglycerol (TAG) metabolismin rats fed high-fat diets containing a FMF obtained by PUFA supplementation to dairy cow diets.Methods: Male Wistar rats were fed (60-d) with S7 (soybean oil, 7%), S30 (soybean oil, 30%), MF30 (soybean oil, 3% + MF,27%) or FMF30 (soybean oil, 3% + FMF, 27%) diets. Biochemicalparameters on serum; TAG levels in liver and skeletal muscle; lipogenic and â-oxidative enzymes activities and expressions in liver;and hepatic TAG secretion rate in vivo were determined. In addition, an oral glucose tolerance test, the metabolites from glycolysisand some key enzyme activities in skeletal muscle were assessed.Statistical differences (p<0.05) were tested by ANOVA.Results: Compared to S7, S30 diet induce TAG accretion in liver (+36%) and skeletal muscle (+61%), decreased muscle glucose utilization by a lower &ux through hexokinase (-53%) and 6-phosphofructokinase (-49%), glucose intolerance and hyperinsulinemia (+48%). #e FMF30 group showed lower levels of TAG in liver (-26%) associated with an enhanced â-oxidation (CPT-I gene expression +122%) and a higher TAG secretion rate (+31%), compared to S30. In muscle, FMF30 also reduced the TAG levels (-49%) and increased the &ux through the 6-phosphofructokinase (+116%) improving the glucose utilization. Conclusions: In conclusion, the FMF prevented the TAG accretion induced by high fat diet consumption and improve the glucose utilization in skeletal muscle.