FUNCTIONAL MILK FAT ATTENUATED THE LIVER OXIDATIVE STRESS IN RATS FED HIGH-FAT DIETS

QUIROZ, NAHUEL; CONTINI, MARíA DEL C.; VERA CANDIOTI, LUCIANA; GERSTNER, CAROLINA; LAVANDERA, JIMENA; SAIN, JULIANA; FARIñA, ANA; GAGLIOSTRO, GERARDO; BERNAL, CLAUDIO; GONZALEZ, MARCELA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias 2017; 2017

Sociedades de Biociencias

Noncommunicable chronic diseases (NCCD) have high prevalence and repercussion inthe morbi-mortality of our population and have been related with dietarydisorders. In this regards, high intake of fat, including Milk Fat (MF), hasbeen associated with lipid alterations, oxidative stress and cardiovasculardisease risk. However, MF can be reduced in the saturated fatty acids andincreased the Conjugated Linoleic Acids (CLA) and vaccenic acid (t11-18:1)contents by unsaturated fatty acid supplementation to dairy cow diets leadingto a functional milk fat (FMF) with beneficial effects on NCCD risk. Since, theanalysis of biomarkers for oxidative stress and lipid alterations is essentialfor the diagnostic and control of certain NCCD, the aim of this study was toinvestigate some of these biomarkers involved in hepatic oxidative stress ofrats fed diets containing MF and FMF at high levels. Male Wistar rats were fed(60-d) with S7 (soybean oil, 7%), S30 (soybean oil, 30%), MF30 (soybean oil, 3%+ MF, 27%) or FMF30 (soybean oil, 3% + FMF, 27%) diets. The ratio of reducedglutathione and oxidized glutathione (GSH/GSSG) by Capillary electrophoresis, lipoperoxidation(LPO) levels by colorimetric reaction of thiobarbituric acid, and the hepatic expressionof catalase (CAT), transcription factor (NFK-â) and cytokines involved in theinduction of inflammatory status (TNF-á, IL1, IL6) by Real Time PCR wereassessed. Statistical differences (p<0.05) were tested by ANOVA or T test. Comparedwith S7, S30 and MF30 diets increased the hepatic biomarkers of oxidativestress: GSH/GSSG 30% and 42%, LPO 27% and 23%, NFK-â 152% and 78%, TNF-á 93%and 239%, and IL6 115% and 221%, respectively. FMF30 decreased all theseparameters reaching similar values to the S7 group. In conclusion, the intakeof a functional milk fat attenuated the liver oxidative stress in rats fedhigh-fat diets, which could contribute to reducing the NCCD risk.