A FUNCTIONAL MILK FAT CONSUMPTION IMPROVED THE GLUCOSE UTILIZATION IN SKELETAL MUSCLE OF WISTAR RATS FED HIGH FAT DIETS

SAIN, JULIANA; GERSTNER, CAROLINA; SCANAROTTI, IGNACIO; FARIÑA, ANA; LAVANDERA, JIMENA; GONZALEZ, MARCELA; GAGLIOSTRO, GERARDO; BERNAL, CLAUDIO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias

High fat diets (HFD) induce several disorders relatedto metabolic syndrome, including steatosis, hepatic lipid alterations andinsulin resistance. These alterations might be attenuated by bioactivecompounds, like rumenic acid (RA). Milk fat (MF) is the major source of RA, andits level can be increased by lipid supplementation of dairy cow diets obtaininga functional milk fat (FMF). The aim of the present study was to investigateparameters related to glucose and triacylglycerol (TAG) metabolism in rats fed HFDcontaining a FMF. Male Wistar rats were fed (60-d) with diets containing 7% or30% of soybean oil (S7 and S30) and 30% of milk fat (MF30) or functional milkfat (FMF30). The following determinations were assessed: biochemical parameterson serum; TAG levels in liver and skeletal muscle; carnitine palmitoyl transferaseI (CPT-I) expression in liver; hepatic TAG secretion rate; oral glucosetolerance test; metabolites from glycolysis and some key enzyme activities inskeletal muscle; and expression of adiponectin in adipose tissue. Statisticaldifferences (p<0.05) were tested by T test (S7 vs. S30) and One Way ANOVA. Comparedto S7, S30 diet induced TAG accretion in liver (+36%) and skeletal muscle(+61%), decreased the flux through hexokinase (-53%) and 6-phosphofructokinase(PFK1, -49%) in skeletal muscle and rats showed hyperinsulinemia (+48%). Also,the expression of adiponectin in adipose tissue was reduced (50 times). TheFMF30 group showed lower levels of TAG in liver (-26%) associated with anenhanced CPT-I gene expression (+122%) and a higher TAG secretion rate (+31%),compared to S30. In muscle, FMF30 reduced the TAG levels (-49%) and increasedthe flux through the PFK1 (+116%) improving the glucose utilization that couldbe related to an enhanced adiponectin expression (20 times) compared to S30. Inconclusion, the FMF prevented the TAG accretion induced by HFD and improved theglucose utilization in skeletal muscle.