REGULATION OF FATTY ACIDS IN ADIPOSE TISSUE OF RATS FED FUNCTIONAL MILK FAT AT HIGH FAT LEVELS

GERSTNER, CAROLINA; LAVANDERA, JIMENA; SAIN, JULIANA; FARIÑA, ANA; GONZALEZ, MARCELA; GAGLIOSTRO, GERARDO; BERNAL, CLAUDIO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias 2017; 2017

Sociedades de Biociencias

Rumenic acidand vaccenic acid, naturally present on dairy products, showed to induce healthbenefits on lipid metabolism, therefore some efforts have been made to increasetheir levels in the milk fat (MF) and obtain a functional milk fat (FMF). Sinceadipose tissue (AT) might play an important role on plasma triacylglyceridemodulation, the aim of this study was to investigate the regulation of fattyacid (FA) in adipose tissue of animals fed a FMF at high fat levels. MaleWistar rats were fed (60-d) with S7 (soybean oil, 7%), S30 (soybean oil, 30%),MF30 (soybean oil, 3% + MF, 27%) or FMF30 (soybean oil, 3% + FMF, 27%) diets. InAT was assessed: weight, lipoprotein lipase (LPL) activity by fluorometricmethod, lipogenic and oxidative expression of enzymes by RT-PCR and FA profileby Gas chromatography. Statistical differences (p<0.05) were tested by ANOVA(1x3) and Student t¡¯ Test. MF30 vs S7 increased the AT weight (+30%), and FMFprevent this effect. Compared with S7, the S30 and MF30 diets increased the AT-LPLactivity (+39% and +32%, respectively), and the mRNA levels of CPT-1 (+110% and+85%), without changes in those of DGAT. The FMF30 group (vs S7) also showed anincreased AT-LPL activity (+100%), but comparing with MF the elevation of CPT-Igene expression was higher (163%) and, additionally, a lower lipogenesis reflectedby reduced DGAT gene expression (-63%) and SCD1 index (c9-16:1/16:0 ratio) (-48%)was observed. Compared to MF30, the FMF30 diet increased the levels of ¦Á-linolenicacid (40%), rumenic acid (183%) and vaccenic acid (+169%). In conclusion, theFMF prevented the AT accretion by a higher b-oxidation/lipog¨¦nesisratio, and these balance could be associated with a significant incorporationof rumenic and vaccenic acids in this tissue.