Immunomudulation of lactobacillus rhamnosus RC007 on the intestinal toxicity of deoxynivalenol

GARCÍA, G; PAYROS, D; DOGI C.A; GRECO C.; CHULZE, S; DE MORENO DE LEBLANC A; CAVAGLIERI L.R.; OSWAL, I

Congreso; 5th Beneficial Microbes Conference will focus on pre- and probiotics for lifelong human and animal health; 2016

The legislation of the European Union banned antibiotics as growth promoters in food animals.In addition, the Food and Agricultural Organization estimates that mycotoxins contaminate 25% of the world?s agricultural commodities. Different alternatives, such as the use of probiotics, have been exploredto stimulate gut health in weaned pigs.Lactobacillus rhamnosusRC007 was previously isolated from maize silageand demonstrated beneficial properties in a mice colitis model. The aim of the present study was to evaluate the immuneeffect on the intestinal toxicity of deoxynivalenol(DON) in anex vivoporcine model.Jejunal explants were obtained from 5 weeks old crossbred castrated malepiglets. The treatmentsevaluated were: 1) porcine jejunal explantsin complete medium, 2) porcine jejunal explants exposed for 3 h to10 μM DON in complete medium, 3) porcine jejunal explantswith109UFC/mlL. rhamnosusRC007 in complete medium, 4) porcine jejunal explants pre-incubated 1 h with 109UFC/ml L. rhamnosusRC007and then exposed for 3 h to 10 μM DON in complete medium. The explants were incubatedat 39°C.For the gene expression analysis, total RNAs wereextracted and then reverse transcription and qPCR steps were performedto determinate IL-1β,TNFα, IL-8 and IL-22. Cellular permeabilitywas measured byaUssing chamber.Treatment of intestinal explants with DONsignificantly increasedthe expression of IL-1β, TNFα, IL-8, IL-22 andincreased the paracellular permeability. By contrast, the pre-incubation with L. rhamnosusRC007reduced the expression of these pro-inflammatorycytokines and the paracellular permeability. In conclusion,L. rhamnosus RC007 is a probiotic candidate to be included as feed additive able to decreases the intestinal toxicity caused by DON.