Glucose metabolism in skeletal muscle of rats fed a functional milk fat at high levels

SAIN, JULIANA; SCANAROTTI, IGNACIO; GERSTNER, CAROLINA; FARIÑA, ANA; LAVANDERA, JIMENA; GONZALEZ, MARCELA; BERNAL, CLAUDIO

Ciudad de Cordoba

Congreso; 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Rumenic acid (RA) could improve the glucose (Glc) uptake and utilizationpreventing insulin resistance. As dairy products are the major source of RA,its content in milk fat has been increased altering the diet of dairy cows. Since,skeletal muscle is the major tissue in Glc utilization our aim was toinvestigate parameters related to Glc metabolism in gastrocnemius muscle (GM) ofrats fed high fat diets containing a functional milk fat. Male Wistar rats werefed (60-d) with high fat diets containing 7% or 30% of soybean oil (S7 and S30)and 30% of milk fat (M30), functional milk fat (F30) or standardized milk fat(E30). Serum insulin levels, Glc and triacylglycerol (TAG) in serum and GM, andkey metabolites levels and enzymes activities from Glc metabolism in GM wereassessed. Statistical differences (p<0.05) were tested by T test (S7 vs.S30)and ANOVA. Compared to S7 diet, S30 increased serum insulin and Glc and TAG contentin GM in parallel with high citrate levels and phosphofructokinase-1 (PFK1)inhibition. This could suggest a lower Glc utilization. In contrast, comparedwith S30 PFK1 activity increased in all milk fats diets but the flux throughPFK1 was higher in F30. Pyruvate levels were lower in F30 and E30 suggesting ahigher metabolization rate. Thus, the Glc metabolism in GM of rats fed high fatdiets depends on the type of fat and the involved mechanisms are different.