INFLUENCE OF DIETS WITH DIFFERENT PROFILE OF UNSATURATED FATTY ACIDS ON DHA CONTENT AND CICLOOXIGENASES EXPRESSION IN BRAIN. EFFECTS OF TRANS FATTY ACID

LAVANDERA, JIMENA; SAIN, JULIANA; REUS, VERÓNICA; FARIÑA, ANA; CARRERA, LARISA; BERNAL, CLAUDIO; GONZALEZ, MARCELA

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA (SAIC); 2016

SAIC

The levels of docosahexaenoic acid (DHA ,22:6n-3), araquidonic acid (AA, 20:4n-6) and antiand pro inflammatory lipids mediators derivated from these polyunsaturatedfatty acid (PUFA) for the action of enzymes, like cicloxygenases are criticalto the structure and normal brain function. Trans fatty acid (TFA) presents inthe diet could be incorporated into neuronal membranes affecting metabolicprocess. The objective was evaluate in mice brain, the effect of diets withdifferent composition of unsaturated FA with or without TFA, on the DHAcontent, TFA retention, cicloxygenases (COX , COX , and transcriptional factorPPAR¦ÃmRNA levels, and also histological brain changes. Male CF1 mice (22g) were fed(120 days) with diets based on the AIN-93G (American Institute of Nutrition)with different MUFA relation n-3/n-6/n-9: Rappessed (R) (10.9/19.0/63.2), Corn(C) (0.9/53.3/31.3) and Olive oil (O); with or without 0,75% TFA: Rt, Ct andOt, respectively. AG composition was determinated by gas chromatography and COX, COX and PPAR¦ÃmRNA levels, by real time PCR. Histological serial sections were stained withhematoxylin-eosine. Result were analyzed by One-Way ANOVA, followed by Scheff¨¦(p<0.05). TFA retention was 0.96 (Ot), 0.81 (Ct) y 0.56 (Rt). DHA levels(%), was higher in animals fed with R vs C and O (20% y 16%, p<0.05), andwas lower the PUFA n-6/n-3 ratio (32% y 29%, p<0.05), respectively. COX2mRNA levels diminished in groups fed O (55%) and R (46%) vs C. PPAR¦Ã was increasedin R (34%, p<0,05) vs O and C. The addition of TFA not modified the mRNAlevels of these parameters. Histological sections showed lymphocytes presencein relation with the ependymal lining cells of the brain surface in animals fedO and C. The results obtained showed that the type of FA in the diet ratherthan TFA supplementation could modulate inflammatory response in brain.