THE EFFECT OF MATERNAL CONJUGATED LINOLEIC ACID AND HIGH FAT DIETS ON THE TRIACYLGLYCEROL METABOLISM IN ADULT MALE RAT OFFSPRING

GONZALEZ, MARCELA; GERSTNER, CAROLINA; LAVANDERA, JIMENA; SAIN, JULIANA; FARIÑA, ANA; JOVELLANO, JULIETA; BERNAL, CLAUDIO

Córdoba

Congreso; 52th annual meeting Argentine for Biochemistry and Molecular Biology; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB)

The aim was to investigatethe potential beneficial effects of maternal CLA consumption at high fat (HF) dieton Triacylglycerol (TAG) metabolism in adult male rat offspring. Female Wistarrats were fed diets containing recommended (7%) or HF (20%) levels supplementedor not with CLA (C7, C20 and CLA20) during 4 weeks before and throughoutpregnancy and lactation. Male offspring of C7, C20 and CLA20 rats were fed withthe same diets of their mothers (C7/C7, C20/C20 and CLA20/CLA20 groups), whileother group of the offspring of CLA20 were fed C20 diet (CLA20/C20) for 9weeks. Serum (S) and hepatic (H) TAG levels, H-TAG secretion rate (TAG-SR), adiposetissue (AT) and muscle (M) LPL activity and lipogenic (FAS, ACC, G6PDH) andoxidative (CPT1) activity and expression were measured. Results: 1) comparingwith C7/C7, C20/C20 rats showed an higher H-TAG (57%) associated with a lowerTAG-SR (-29%). In addition, S-TAG (-17%), AT-LPL activity (-37%), H-lipogenicenzyme activities and expressions were reduced; 2) CLA20/C20 rats tended tonormalize S-TAG and H-TAG levels associated with a normal TAG-SR; 3) Adultoffspring consuming CLA at HF levels (CLA20/CLA20) restored the S-TAG and H-TAGlevels in parallel to normal TAG-SR and improved AT-LPL activity. Maternal CLAconsumption metabolically may contribute to prevent or attenuate the different TAGalterations of adult offspring at HF levels.