Biological Signals Induced by Probiotics in the Gut Immune Stimulation

CAROLINA MALDONADO GALDEANO; ALEJANDRA DE MORENO DE LEBLANC; CECILIA DOGI; ESTEBAN CARMUEGA; RICARDO WEILL; GABRIELA PERDIGÓN.

Boston

Congreso; 14th International Congress of Mucosal Immunology; 2009

Probiotic bacteria and probiotic fermented milk (PFM) induce gut stimulation increasing cytokine+ cells with NFAT transcriptional factor activation. Other biological signals to activate the intestinal cells by probiotics were analyzed. Methods: Intestinal epithelial (IEC) and Peyer patches (PP) cells were isolated from intestine of mice given the probiotic Lactobacillus casei CRL431 (Lc) for 7 days or PFM for 5 days. Anti-mouse NF-kappa-B antibody was used to block this via in cell cultures priorprobiotic stimulation. IL-6 and TNF-alpha were determined in the culture supernatants by ELISA. Co-stimulatory CD-80, CD-86 and HLA-II molecules were analyzed by flow citometry in PP cells from animals fed with Lc or PFM. Results: PFM stimulation increased IL-6 production by IEC. PP cells released TNF-alpha when they were stimulated with PFM or Lc. The production of both cytokines was dependent of NF-kappa-B pathway. Increases of co-stimulatory and HLA molecules expression by probiotic or PFM administration were not observed. Conclusions: Probiotics or PFM activate NF-kappa-B via in IEC and PP cells to promote the production of certain cytokines. Probiotics do not affect the expression of co-stimulatory molecules in gut immune cells. These results agree with previous report where probiotics administration activated gut innate immunity without antigenic presentation.