NUTRITIONAL INTERACTION BETWEEN TRANS FATTY ACIDS AND UNSATURATED FATTY ACODS FROM DIFFERENT SOURCES ON REGULATION OF TRIGLICERIDE METABOLISM

GONZALEZ, MARCELA; SAIN, JULIANA; LAVANDERA, JIMENA; BERNAL, CA

granada

Congreso; 20 th International Congress of Nutrition; 2013

Federación of European Nutrition Societies (FENS)

Background and objectives: the dietary fat intake modulates the plasma and tissue levels of triglycerides (TG). The aim of this study was to evaluate the effect of diets with different unsaturated fatty acid (UFA) in the presence or absence of trans fatty acids (TFA) on regulation of triglyceride metabolism in mice. Methods: Male CF1 mice (22g) were fed (30 days) with a standard diets differing in dietary n-3/n-6/n-9 UFA ratios: Canola (CO: 10.9/19.0/63.2), Maize (MO: 0,9/53.3/31.3) or Olive (OO: 0,8/9,7/76,3) oils containing 0,75% of TFA (Cot, Mot and OOt, respectively) or not. Serum and liver TG content, hepatic TG-pre-a-lipoprotein secretion rate (TGSR), and lipoprotein lipase (LPL) activity from adipose tissue and muscle were determined. Results: TFA induced hepatic steatosis (+100% OOt vs. OO and Cot vs. CO) and increased TGSR in OOt and Cot groups (+260% in OOt vs. OO and +160% in Cot vs. CO). However, an increasing LPL activity in adipose tissue was observed (+200% in OOt vs. OO and +167% on Cot vs. CO), leading to a normal levels of serum TG in these groups. In animals fed Mot, no changes were observed in the evaluated parameters. Conclusions: TFA altered metabolism depending on the type of  dietary UFA. Adipose tissue could play a key role in the plasma TG regulation, however the TGSR did not normalize the hepatic TG levels.