INVESTIGADORES
FERNANDEZ Juan Manuel
congresos y reuniones científicas
Título:
STRONTIUM RANELATE PREVENTS THE DELETERIOUS ACTION OF ADVANCED GLYCATION END PRODUCTS ON BONE MARROW PROGENITOR CELLS VIA ACTIVATION OF WNT PATHWAY
Autor/es:
FERNANDEZ JM; MOLINUEVO MS; SCHURMAN L; SEDLINSKY C; MCCARTHY A
Reunión:
Congreso; IOF (international Osteoporosis Foundation Regionals Brazil´12 and 1º Latin America Osteoporosis Meeting; 2012
Resumen:
Aims/Objetivos/Objetivo: To evaluate the ability of strontium ranelate (SR) to prevent AGEs deleterious action on the
osteoblastic differentiation of bone marrow progenitor cells (BMPC) in vitro. We also investigated the role of Wnt pathway
and calcium channels in these possible actions of SR.
Methods/Métodos/Métodos: BMPC were obtained from Sprague-Dawley young male rats by flushing their femoral
diaphysary central cavity with DMEM. Cells were cultured in an incubator at 37ºC, 95% humidity and 5% CO2. After they
reached confluence, the BMPC monolayer was trypsinized and cells were seedeed in adequate tissue culture plates. For
osteoblastic differentiation, BMPC were further cultured in DMEM plus sodium b-glycerophosphate and ascorbic acid in
the presence of 10μg/ml of either AGEs or non glycated serum abumin (BSA), with or without 0.1mM SR, for 15
days.BMPC differentiaton was evaluated assesing alkaline phosphatase activity (ALP) using p-nitrophenylphosphate as
substrate; and collagen extracellular production by Sirius red stain. The phopshorylation of b-catenin, a key mediator of
the Wnt pathway, was assessed by indirect inmunofluorescence.
Results: We found that SR co-incubated with BSA, increases osteoblastic differentiation of BMPC by increasing both
ALP activity (125% vs BSA, p