EFFECT OF BIOACTIVE COMPOUNDS DERIVED FROM GRAPE POMACE ON OBESITY-RELATED KIDNEY INJURY

FIGUERAS TS; GARCIA RD; PERDICARO DJ; MUSCIA V; CACCIAMANI V; VALLES PG; VAZQUEZ MA; COSTANTINO V

Congreso; Reunion Anual de Sociedades de Biociencias; 2022

Obesity is an important risk factor for the kidney disease development. Epidemiological studies have shown that the prevalence ofchronic kidney disease is increasing concomitant with obesity. Thephysiopathology of obesity-related kidney disease is multifactorial.Several mechanisms by which lipids can cause kidney damagehave been proposed, including inflammation, oxidative stress, andfibrosis. The intake of foods rich in bioactive compounds, such aspolyphenols attenuates pathologies associated with obesity andreduces inflammation and oxidative stress. Grape pomace extract(GPE) is obtained from grape pomace, a residue of the winemaking process. GPE is mainly constituted of berry skins and seeds,which still contain high amounts of phenolic compounds. Given thatin Mendoza, the wine industry each year generates great amountsof pomace, this can be used for implementing strategies in the prevention of obesity-associated pathologies. We propose to evaluatethe GPE effect in renal injury related to obesity. For this, C57BL/6Jmice (20–25 g) were divided into 4 groups (n = 8 each) and fedfor 10 weeks as follows: 1) Control group (Ctrl): standard diet; 2)Ctrl group + diet supplemented with GPE 300 mg/Kg of weight/day;3) HFD group (high fat), and 4) HFD + GPE 300 mg/Kg of weight/day. At the end, blood samples were taken, adipose tissue was removed and the mice were nephrectomized to obtain tissue samples.The concentrations of biochemical parameters were analyzed using commercial kits. We shown that GPE supplementation significantly reduced body weight, visceral and perirenal adipose tissuein the HDF+GPE group compared to the HFD group. In addition,the biochemical parameters concentrations such as cholesterol andserum urea were significantly decreased in the HDF+GPE groupcompared to the HFD group. These results highlight the potentialuse of grape-derived bioactive compounds to reduce obesity-relatedkidney injury.