Effect of maternal CLA consumption on liver triacylglycerol regulation of adult offspring

LAVANDERA, JIMENA; GERSTNER, CAROLINA; FARIÑA, ANA; SAIN, JULIANA; GONZALEZ, MARCELA; BERNAL, CLAUDIO

Roma

Congreso; 5th European Nutrition and Dietetics Conference; 2016

Nutritional Conference

Animal studies reveal that Conjugated linoleicacid (CLA) has beneficial effects against cancer, obesity, diabetes, inflammationand atherosclerosis. However, CLA was associated with liver steatosis in mice,while detrimental and protective effects on fatty liver have been shown inrats. Since there is an association between maternal nutrition and risk of offspringchronic disease, the aim was to investigate the potential effects of maternalCLA consumption on the liver regulation of triacylglycerol accretion of adultoffspring. Female Wistar rats were fed control (C) or CLA-supplemented diet(CLA) during 4 weeks before and throughout pregnancy and lactation. Maleoffspring of CLA rats were fed C (CLA/C) or CLA (CLA/CLA) diets, while maleoffspring of C rats were fed C (C/C) diet (9 weeks). Hepatic triacylglycerol contentand the activity and expression of lipogenic and oxidative enzymes weremeasured. Comparing with C/C, CLA/CLA rats showed a tendency for decreasedtriacylglycerol depots (-22%) associated with a significant reduction of FAS (-37%)and ACC (-27%) enzyme activities, as well as of the mRNA levels of FAS (-44%),ACC (-48%), SCD (-54%) and SREBP1c (-25%), without changes in B-oxidationparameters. CLA/C rats did not show changes in hepatic triacylglycerol levels,but the FAS (-38%) and ACC (-31%) activities, as well as the expression of ACC (-62%)were significantly diminished, associated to a lower expresion of the keyenzyme of B-oxidation (CPT-Ia) (-23%), PPARA (-22%) and PPARA (-45%). MaternalCLA consumption could metabolically contribute to prevent or attenuate the riskof fatty liver of adult offspring.