Mass production studies in solid substrates with the entomopathogenic fungus, Purpureocillium lilacinum

BARRA, P.; BARROS, G.; ETCHEVERRY, M.; NESCI, A.

International Journal of Advance Agricultural Research

Bluepen journals

Año: 2018 vol. 6 p. 78 - 84

2053-1265

The objective of this study was to evaluate several substrates to determine theoptimal condition of entomopathogenic mass production with the purpose ofdeveloping a mycoinsecticide to be used for an integrated pest management.Conidial quality and yield for two isolates of Purpureocillium lilacinum weredetermined. For quality parameters assessment, germination and puritypercentage and viability of conidia were determined. For the productionparameters, the conidial yield was calculated. The main results obtained showthe type of substrate that had the greatest effect on the viability, germination andyield of conidia of P. lilacinum. The substrate that presented the highestproduction of conidia per gram was rice, showing 2.1×109 for isolate JQ926212and 1.6×109 for JQ926223. In the particulate substrates, the percentage puritywas about 99%, while in powder substrate, the percentage purity was low withvalues ranging from 23 to 75%. The highest percentage of moisture content wasabout 40% for rice and wheat, while for maize meal and vermiculite thepercentage was close to 20%. In conclusion, P. lilacinum can be mass multipliedin rice grains and vermiculite.