The structure of the extended E2 DNA-binding domain of the bovine papillomavirus-1

LEROY, LUDMILA; BARBOSA, JOÃO ALEXANDRE RIBEIRO GONÇALVES; DE PRAT-GAY, GONZALO; POLIKARPOV, IGOR; PINHEIRO, CARLOS BASÍLIO

PROTEINS: STRUCTURE, FUNCTION AND GENETICS

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2020 vol. 88 p. 106 - 112

0887-3585

Bovine papillomavirus proteins were extensively studied as a prototype for the human papillomavirus. Here, the crystal structure of the extended E2 DNA-binding domain of the dominant transcription regulator from the bovine papillomavirus strain 1 is described in the space group P3121. We found two protein functional dimers packed in the asymmetric unit. This new protein arrangement inside the crystal led to the reduction of the mobility of a previously unobserved loop directly involved in the protein-DNA interaction, which was then modeled for the first time.