Potentiation of aflatoxin B1 adsorbant effect of a commercial additive mixed 1 with different probiotic yeasts strains

POLONI, V.; DOGI C.A; PEREYRA, C; FERNANDEZ JURI, G; KOHLER, P; DALCERO, A; CAVAGLIERI L.R.

FOOD ADDITIVES AND CONTAMINANTS

TAYLOR & FRANCIS LTD

Año: 2015 vol. 32 p. 970 - 976

0265-203X

The aim of study was to potentiate the AFB1 adsorbant effect of an animal feed commercial additive (CA), which contains inactive lysate of three S. cerevisiae strains, active enzymes, adsorbents and a selenium-amino acid complex, when was mixed separately with three S. cerevisiae strains. Twenty and 50 ng g-1 of AFB1 were used to determine the binding capacity of different concentrations of the CA alone and in presence of the yeasts strains under gastrointestinal conditions as well as the toxin desorption. Viability of yeasts in presence of CA was evaluated. Results showed that the CA did not affect the viability of the yeasts strains assayed. S. cerevisiae RC012 and S. cerevisiae RC016 showed the highest adsorption percentages, while RC009 showed the lowest. The CA alone showed low adsorption percentages compared to yeasts strains at both toxin concentrations. At 20 ng g-1, CA was significantly efficient adsorbing AFB1 only when was combined with RC016 strain. In general, the mixture of the CA with the rest of the yeasts strains produced an adsorbent effect equal or less than the produced by the CA alone. RC016 strain alone did not show desorption percentages, whereas the rest of the yeasts assayed showed desorption percentages under 20%. Desorption of AFB1 by the CA alone and in combination with the yeasts raised along the CA increased. At 50 ng g-1, the CA alone was significantly less efficient adsorbing the toxin than in combination with the yeasts; at 25% of the CA, RC012 showed the highest adsorption percentage. The RC016 strain presented high adsorption percentages at 25 and 50%. Desorption at 50 ng g-1 showed the same behavior that at 20 ng g-1 of AFB1. When the AFB1 concentration to be adsorbed in the assays increased, the mixtures of CA plus yeasts strains were more efficient. Results demonstrated the improvement of the CA in AFB1 adsorption once is mixed with the live yeasts assayed.