Induction of nitric oxide synthase activity in adrenal cells.

CYMERYNG CB, LOTITO SP, COLONNA C, CORNEJO MF, PODESTÁ EJ.

ENDOCRINE RESEARCH

TAYLOR & FRANCIS INC

Año: 2000 p. 589 - 595

0743-5800

The induction of nitric oxide synthase (NOS) II by bacterial lypopolysaccharide (LPS) was studied in a steroidogenic mouse tumor adrenal cell line (Y1). Conditioned media from LPS-stimulated peritoneal macrophages induced an increase in NOS II expression as shown by western and northern blot analysis. Accordingly, in the presence of conditioned media an increase in nitrite levels was observed. In addition, steroid production was significantly decreased. In conclusion, NOS II expression could be induced in steroidogenic cells with a concomitant inhibition of steroid production