INVESTIGADORES
NESCI Andrea Veronica
artículos
Título:
Biological interacitons to select biocontrol agents against toxigenic strains of Aspergillus flavus and Fusarium verticillioides from maize
Autor/es:
MIRIAM G. ETCHEVERRY; ANDREA SCANDOLARA; ANDREA NESCI; MARTA SOFIA VILAS BOAS RIBEIRO; PAOLA PEREIRA; PAOLA BATTILANI
Revista:
MYCOPATHOLOGIA
Editorial:
Kluwer Academic Publishers
Referencias:
Lugar: The Netherland; Año: 2009 vol. 167 p. 287 - 295
ISSN:
0301-486X
Resumen:
Biological control represent an alternative
to the use of pesticides in crop protection. A key to
progress in biological control to protect maize against
Fusarium verticillioides and Aspergillus flavus maize
pathogens are, to select in vitro, the best agent to be
applied in the field. The aim of this study was to
examine the antagonistic activity of bacterial and yeast
isolates against F.verticillioides and A. flavus toxigenic
strains. The first study showed the impact of Bacillus
amyloliquefaciens BA-S13, Microbacterium oleovorans
DMS 16091, Enterobacter hormomaechei
EM-562T, and Kluyveromyces spp. L14 and L16
isolates on mycelial growth of two strains of A. flavus
MPVPA 2092, 2094 and three strains of F. verticillioides
MPVPA 285, 289, and 294 on 3% maize meal
extract agar at different water activities (0.99, 0.97,
0.95, and 0.93). From this first assay antagonistics
isolates M. oleovorans, B. amyloliquefaciens and
Kluyveromyces sp. (L16) produced an increase of lag
phase of growth and decreased a growth rate of all
fungal strains. These isolates were selected for futher
studies. In vitro non-rhizospheric maize soil (centrally
and sprayed inoculated) and in vitro maize (ears apex
and base inoculated) were treated with antagonistics
and pathogenic strains alone in co-inoculated cultures.
Bacillus amyloliquefaciens significantly reduced
F. verticillioides and A. flavus count in maize soil
inoculated centrally. Kluyveromyces sp. L16 reduced
F. verticillioides and A. flavus count in maize soil
inoculated by spray. Kluyveromyces sp. L16 was the
most effective treatment limiting percent infections by
F. verticillioides on the maize ears.