INVESTIGADORES
DE PRAT GAY Gonzalo
artículos
Título:
Association of complementary fragments and the elucidation of protein folding pathways
Autor/es:
PRAT GAY G. DE
Revista:
PROTEIN ENGINEERING
Referencias:
Año: 1996 vol. 9 p. 843 - 847
ISSN:
0269-2139
Resumen:
Natural or engineered sites for chemical cleavage can be used to generate complementary fragments of well characterized proteins. The peptide fragments represent a unique tool for studying early events in protein folding, since these are usually the most inaccessible to the experimentalist. Analysis of the isolated fragments in non-denaturing conditions together with the determination of the structure of the folded non-covalent complexes and, most importantly, the kinetic analysis of the resulting second-order association/folding reaction can give a more complete picture of a folding pathway. The contribution of fragments to the understanding of some well characterized protein folding pathways is discussed.
