Biological signals induced by probiotics in the gut immune stimulation

MALDONADO GALDEANO, CAROLINA; DE MORENO DE LEBLANC ALEJANDRA; DOGI CECILIA; CARMUEGA ESTEBAN; WEILL RICARDO; PERDIGÓN GABRIELA

MUCOSAL IMMUNOLOGY

NATURE PUBLISHING GROUP

Año: 2009 vol. 2 p. 1 - 1

1933-0219

Probiotic bacteria and probiotic fermented milk (PFM) induce gut stimulation increasing cytokine+ cells with NFAT transcriptional factor activation. Other biological signals to activate the intestinal cells by probiotics were analyzed. Methods: Intestinal epithelial (IEC) and Peyer patche´s (PP) cells were isolated from intestine of mice given the probiotic Lactobacillus casei CRL431 (Lc) for 7 days or PFM for 5 days. Anti-mouse NFkB antibody was used to block this via in cell cultures prior-probiotic stimulation. IL-6 and TNFa were determined in the culture supernatants by ELISA. Co-stimulatory CD-80, CD-86 and HLA-II molecules were analyzed by flow citometry in PP cells from animals fed with Lc or PFM. Results: PFM stimulation increased IL-6 production by IEC. PP cells released TNFa when they were stimulated with PFM or Lc. The production of both cytokines was dependent of NFkB pathway. Increases of co-stimulatory and HLA molecules expression by probiotic or PFM administration were not observed. Conclusions: Probiotics or PFM activate NFkB via in IEC and PP cells to promote the production of certain cytokines. Probiotics do not affect the expression of co-stimulatory molecules in gut immune cells. These results agree with previous report where probiotics administration activated gut innate immunity without antigenic presentation.