Saccharomyces cerevisiae strains reduce Aspergillus parasiticus growth and aflatoxin B1 production at different interacting environmental conditions, in vitro

ARMANDO, MR; DOGI C.A; ROSA C.A.R.; DALCERO A.M.; CAVAGLIERI L.R.

FOOD ADDITIVES AND CONTAMINANTS

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2012 vol. 29 p. 1443 - 1449

0265-203X

The effect of Saccharomyces cerevisiae RC008 and RC016, previously selected based on their aflatoxin B1 binding ability and beneficial properties, against Aspergillus parasiticus under different interacting environmental conditions was evaluated. Studies on the lag phase, growth rate and aflatoxin B1 production were carried out in vitro under different regimes of aw (0.95 and 0.99); pH (4 and 6); temperature (25 and 37°C) and oxygen availability (normal and reduced). Both yeast strains showed great antagonistic activity at pH 4, decreasing growth rate compared to the control. The RC008 strain showed the greatest inhibitory activity at all assayed conditions. Aspergillus parasiticus was able to produce large amounts of AFB1 in vitro. A significant decrease of AFB1 levels in comparison with the control were observed with yeast interaction. Differences between control and treatment values ranged between 130 and 5400 ng/ml. Saccharomyces cerevisiae RC008 and RC016 could be considered as effective agents to reduce growth and AFB1 production at different interacting environmental conditions, related to that found in stored feedstuff. The importance of the present work lays in the search for live strains with both probiotic and bio-control properties, able to prolong the safe storage of feedstuff and exert beneficial properties after animal consumption, to be included in a novel product to animal feed.