In vitro study on the effect of Saccharomyces cerevisiae strains on growth and mycotoxins production by Aspergillus carbonarius and Fusarium graminearum

ARMANDO, MR; DOGI C.A; POLONI, V.; DA ROCHA ROSA, C; DALCERO A.M.; CAVAGLIERI L.R.

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 161 p. 182 - 188

0168-1605

The effect of Saccharomyces cerevisiae RC008 and RC016, previously selected based on their aflatoxin B1 mycotoxin binding ability and beneficial properties, against Aspergillus carbonarius and Fusarium graminearum under different interacting environmental conditions was evaluated. In vitro studies on the lag phase, growth rate and ochratoxin A/zearalenone production were carried out under different regimes of aw (0.95 and 0.99); pH (4 and 6); temperature (25 and 37°C) and oxygen availability (normal and reduced). Both yeast strains showed antagonistic activity, decreasing growth rate compared to the control. The RC016 strain showed the greatest inhibitory activity at all assayed conditions. Aspergillus carbonarius and Fusarium graminearum were able to produce large amounts of mycotoxins in vitro. A significant decrease of mycotoxins levels in comparison with the control were observed with yeast interaction. .Saccharomyces cerevisiae RC008 and RC016 could be considered as effective agents to reduce growth and OTA and ZEA production at different interacting environmental conditions, related to that found in stored feedstuff. The use of live strains with both beneficial and bio-control properties, able to prolong the safe storage of feedstuff and exert beneficial properties after animal consumption, are important to be included in a novel product to animal feed. The use of S. cerevisiae RC008 and RC016 as additives of animal feed  could be an alternative to control mycotoxigenic fungi in stored feedstuff.