Statistical optimization of culture conditions for biomass production of probiotic gut-borne Saccharomyces cerevisiae strain able to reduce fumonisin B1

ARMANDO, MR; GALVAGNO, M; DOGI C.A; CERRUTI, P; DALCERO A.M.; CAVAGLIERI L.R.

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2013 vol. 114 p. 1338 - 1346

1364-5072

Aim: to evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B1 (FB1) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology. Methods and results: Using Plackett?Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (g l−1): fermentable sugars provided by sugar cane molasses (CMs), yeast extract (YE) and (NH4)2HPO4 (DAP) was formulated. The S. cerevisiae RC016 strain showed the greatest binding at all assayed FB1 concentration. The CMs, YE, DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC016. Conclusion: A combination of CMs 17%; YE 4.61 g L-1 and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC016. Significance and Impact of the Study: The importance of the present work lays in the search for live strains with both probiotic and fumonisin B1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed.