MATERNAL CONJUGATED LINOLEIC ACID CONSUMPTION PREVENTED TRIACYLGLYCEROL ALTERATIONS INDUCED BY A HIGH-FAT DIET IN MALE ADULT RAT OFFSPRING

GONZALEZ, MARCELA; LAVANDERA, JIMENA; GERSTNER, CAROLINA; FARIÑA, ANA CLARA; SAIN, JULIANA; BERNAL, CLAUDIO

BRITISH JOURNAL OF NUTRITION

CAMBRIDGE UNIV PRESS

Lugar: Cambridge; Año: 2020 vol. 124 p. 286 - 295

0007-1145

Maternal nutritional programming by a high-fat (HF) diet is related to hepatic lipid accumulation and 44 steatosis in offspring. Conjugated linoleic acid (CLA) might ameliorate impaired hepatic lipid homeostasis; therefore, the aim was to investigate the potential preventive effect of maternal CLA consumption on triacylglycerol (TAG) metabolism alterations induced by HF diets in adult male rat offspring receiving or not receiving CLA. Female Wistar rats were fed a control (C) diet, HF diet or HF diet supplemented with CLA (HF+CLA) for 4 weeks before mating and throughout pregnancy and lactation. After weaning, for 9 weeks, male offspring of C or HF rats continued with the same diets as their mothers, and male offspring of HF+CLA rats were fed HF or HF+CLA diets. Nutritional parameters, serum and liver TAG levels, the TAG secretion rate, and the activities as well as gene expression of key hepatic enzymes involved in TAG regulation were assessed.In offspring, our results showed that serum and liver TAG levels were increased in the HF/HF group and normalized in offspring groups of CLA-fed mothers. Maternal CLA prevented hepatic TAG accumulation by increasing hepatic TAG secretion and decreasing epididymal white adipose tissue (EWAT) weight in both offspring groups of CLA-fed mothers. The most interesting finding was that the modulating action of CLA on liver and EWAT TAG accretion remained even in the absence of CLA in the offspring diet. We hypothesize that an early-life CLA programming mechanism on lipid depots, in particular, by preventing liver steatosis, may be involved.