INVESTIGADORES
PIROLA Carlos Jose
artículos
Título:
Gene-gene interaction between serotonin transporter
Autor/es:
SILVIA SOOKOIAN; TOMAS FERNANDEZ GIANOTTI; ADRIANA BURGUEÑO; CARLOS JOSE PIROLA
Revista:
CHRONOBIOLOGY INTERNATIONAL
Editorial:
TAYLOR & FRANCIS INC
Referencias:
Año: 2010 vol. 6 p. 1202 - 1218
ISSN:
0742-0528
Resumen:
Serotonergic neurotransmission and the master circadian CLOCK gene are physiological
modulators of the circadian system. In addition, both are involved in the physiopathology
of metabolic syndrome (MS). The authors sought to examine the potential
effect of the gene-gene interaction between the functional 44-bp insertion/deletion
polymorphism in the promoter region (serotonin-transporter-linked promoter region
polymorphism or 5-HTTLPR) of the serotonin transporter gene (SLC6A4) and
common variants of the gene CLOCK on the genetic risk underlying MS of shiftworkers.
To test this hypothesis, 856 men were studied; 518 dayworkers were compared
with 338 rotating shiftworkers. Medical history, health examination including
anthropometric and arterial blood pressure measurements, a questionnaire on
health-related behaviors, and biochemical determinations were obtained from every
participant. 5-HTTLPR genotypes were determined using polymerase chain reaction
followed by gel electrophoresis. Six tag single-nucleotide polymorphisms (SNPs) in
the CLOCK gene with a minor allele frequency >10 % (rs1554483 C/G, rs11932595
A/G, rs4580704 C/G, rs6843722 A/C, rs6850524 C/G, and rs4864548 A/G), encompassing
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
A/G, rs4580704 C/G, rs6843722 A/C, rs6850524 C/G, and rs4864548 A/G), encompassing
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
To test this hypothesis, 856 men were studied; 518 dayworkers were compared
with 338 rotating shiftworkers. Medical history, health examination including
anthropometric and arterial blood pressure measurements, a questionnaire on
health-related behaviors, and biochemical determinations were obtained from every
participant. 5-HTTLPR genotypes were determined using polymerase chain reaction
followed by gel electrophoresis. Six tag single-nucleotide polymorphisms (SNPs) in
the CLOCK gene with a minor allele frequency >10 % (rs1554483 C/G, rs11932595
A/G, rs4580704 C/G, rs6843722 A/C, rs6850524 C/G, and rs4864548 A/G), encompassing
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
A/G, rs4580704 C/G, rs6843722 A/C, rs6850524 C/G, and rs4864548 A/G), encompassing
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
common variants of the gene CLOCK on the genetic risk underlying MS of shiftworkers.
To test this hypothesis, 856 men were studied; 518 dayworkers were compared
with 338 rotating shiftworkers. Medical history, health examination including
anthropometric and arterial blood pressure measurements, a questionnaire on
health-related behaviors, and biochemical determinations were obtained from every
participant. 5-HTTLPR genotypes were determined using polymerase chain reaction
followed by gel electrophoresis. Six tag single-nucleotide polymorphisms (SNPs) in
the CLOCK gene with a minor allele frequency >10 % (rs1554483 C/G, rs11932595
A/G, rs4580704 C/G, rs6843722 A/C, rs6850524 C/G, and rs4864548 A/G), encompassing
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
A/G, rs4580704 C/G, rs6843722 A/C, rs6850524 C/G, and rs4864548 A/G), encompassing
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p=
To test this hypothesis, 856 men were studied; 518 dayworkers were compared
with 338 rotating shiftworkers. Medical history, health examination including
anthropometric and arterial blood pressure measurements, a questionnaire on
health-related behaviors, and biochemical determinations were obtained from every
participant. 5-HTTLPR genotypes were determined using polymerase chain reaction
followed by gel electrophoresis. Six tag single-nucleotide polymorphisms (SNPs) in
the CLOCK gene with a minor allele frequency >10 % (rs1554483 C/G, rs11932595
A/G, rs4580704 C/G, rs6843722 A/C, rs6850524 C/G, and rs4864548 A/G), encompassing
117 kb of chromosome 4 and representing 115 polymorphic sites (r2 > .8),
were genotyped. A significant interaction between the 5-HTTLPR variant and the
haplotype rs1554483rs4864548 of the CLOCK gene was detected for diastolic ( p