INVESTIGADORES
WOLMAN Federico Javier
artículos
Título:
Rapid neutral protease purification by dye-affinity
Autor/es:
FEDERICO J. WOLMAN; MARIANO GRASSELLI; OSVALDO CASCONE
Revista:
PROCESS BIOCHEMISTRY
Editorial:
Elsevier
Referencias:
Lugar: Amsterdam; Año: 2006 vol. 41 p. 356 - 361
ISSN:
0032-9592
Resumen:
Abstract
Dye-affinity hollow fibres were prepared by radiation-induced grafting of polymers with different hydrophilicity degree on to the
membranes and dye attachment after reaction of the grafted epoxy groups with ammonia to produce amino groups. Membranes grafted with
glycidyl methacrylate/dimethyl acrylamide (GMA/DMAA) 1/3 showed better chromatographic properties than those grafted with GMA. The
dye Yellow HE-4R was selected for purification of a neutral protease from FlavourzymeTM on the basis of the maximum capacity of the
membranes obtained. No leakage of the dye from the membranes was evidenced after incubation in 8 M urea in 0.5 M NaOH for 2 months.
From isotherms developed with membranes grafted with GMA/DMAA 1/3, a maximum capacity of 24220 U/ml membrane and a dissociation
constant of 91 U/ml was calculated. Elution with 2 M NaCl at pH 4 allowed quantitative recovery of the adsorbed protease. A yield of 72%
with a purification factor of 3.7 was achieved by working at 5 ml/min for the load, wash and regeneration steps and 1 ml/min for the elution
step. Under these conditions, productivity was 1828 U/h ml, far higher than that attained with the same dye attached to a soft gel: 304 U/h ml
[Iannucci NB, Navarro del Canizo AA, Cascone O. Purification of neutral protease by dye-affinity chromatography. Appl Biochem
Biotechnol 2003;104:17384].
membranes obtained. No leakage of the dye from the membranes was evidenced after incubation in 8 M urea in 0.5 M NaOH for 2 months.
From isotherms developed with membranes grafted with GMA/DMAA 1/3, a maximum capacity of 24220 U/ml membrane and a dissociation
constant of 91 U/ml was calculated. Elution with 2 M NaCl at pH 4 allowed quantitative recovery of the adsorbed protease. A yield of 72%
with a purification factor of 3.7 was achieved by working at 5 ml/min for the load, wash and regeneration steps and 1 ml/min for the elution
step. Under these conditions, productivity was 1828 U/h ml, far higher than that attained with the same dye attached to a soft gel: 304 U/h ml
[Iannucci NB, Navarro del Canizo AA, Cascone O. Purification of neutral protease by dye-affinity chromatography. Appl Biochem
Biotechnol 2003;104:17384].
membranes obtained. No leakage of the dye from the membranes was evidenced after incubation in 8 M urea in 0.5 M NaOH for 2 months.
From isotherms developed with membranes grafted with GMA/DMAA 1/3, a maximum capacity of 24220 U/ml membrane and a dissociation
constant of 91 U/ml was calculated. Elution with 2 M NaCl at pH 4 allowed quantitative recovery of the adsorbed protease. A yield of 72%
with a purification factor of 3.7 was achieved by working at 5 ml/min for the load, wash and regeneration steps and 1 ml/min for the elution
step. Under these conditions, productivity was 1828 U/h ml, far higher than that attained with the same dye attached to a soft gel: 304 U/h ml
[Iannucci NB, Navarro del Canizo AA, Cascone O. Purification of neutral protease by dye-affinity chromatography. Appl Biochem
Biotechnol 2003;104:17384].
membranes obtained. No leakage of the dye from the membranes was evidenced after incubation in 8 M urea in 0.5 M NaOH for 2 months.
From isotherms developed with membranes grafted with GMA/DMAA 1/3, a maximum capacity of 24220 U/ml membrane and a dissociation
constant of 91 U/ml was calculated. Elution with 2 M NaCl at pH 4 allowed quantitative recovery of the adsorbed protease. A yield of 72%
with a purification factor of 3.7 was achieved by working at 5 ml/min for the load, wash and regeneration steps and 1 ml/min for the elution
step. Under these conditions, productivity was 1828 U/h ml, far higher than that attained with the same dye attached to a soft gel: 304 U/h ml
[Iannucci NB, Navarro del Canizo AA, Cascone O. Purification of neutral protease by dye-affinity chromatography. Appl Biochem
Biotechnol 2003;104:17384].
membranes obtained. No leakage of the dye from the membranes was evidenced after incubation in 8 M urea in 0.5 M NaOH for 2 months.
From isotherms developed with membranes grafted with GMA/DMAA 1/3, a maximum capacity of 24220 U/ml membrane and a dissociation
constant of 91 U/ml was calculated. Elution with 2 M NaCl at pH 4 allowed quantitative recovery of the adsorbed protease. A yield of 72%
with a purification factor of 3.7 was achieved by working at 5 ml/min for the load, wash and regeneration steps and 1 ml/min for the elution
step. Under these conditions, productivity was 1828 U/h ml, far higher than that attained with the same dye attached to a soft gel: 304 U/h ml
[Iannucci NB, Navarro del Canizo AA, Cascone O. Purification of neutral protease by dye-affinity chromatography. Appl Biochem
Biotechnol 2003;104:17384].
TM on the basis of the maximum capacity of the
membranes obtained. No leakage of the dye from the membranes was evidenced after incubation in 8 M urea in 0.5 M NaOH for 2 months.
From isotherms developed with membranes grafted with GMA/DMAA 1/3, a maximum capacity of 24220 U/ml membrane and a dissociation
constant of 91 U/ml was calculated. Elution with 2 M NaCl at pH 4 allowed quantitative recovery of the adsorbed protease. A yield of 72%
with a purification factor of 3.7 was achieved by working at 5 ml/min for the load, wash and regeneration steps and 1 ml/min for the elution
step. Under these conditions, productivity was 1828 U/h ml, far higher than that attained with the same dye attached to a soft gel: 304 U/h ml
[Iannucci NB, Navarro del Canizo AA, Cascone O. Purification of neutral protease by dye-affinity chromatography. Appl Biochem
Biotechnol 2003;104:17384].
# 2005 Published by Elsevier Ltd.2005 Published by Elsevier Ltd.
Keywords: Neutral protease; Purification; Hollow-fibre membrane; Affinity chromatographyNeutral protease; Purification; Hollow-fibre membrane; Affinity chromatography