INVESTIGADORES
WOLMAN Federico Javier
artículos
Título:
Protein adsorption onto tentacle cation-exchange hollow-fiber membranes
Autor/es:
CAMPERI, SILVIA A., NAVARRO DEL CAñIZO, AGUSTíN A., WOLMAN, FEDERICO J., SMOLKO, EDUARDO E., CASCONE OSVALDO, GRASSELLI, MARIANO
Revista:
BIOTECHNOLOGY PROGRESS
Editorial:
American Chemical Society and American Institute of Chemical Engineers
Referencias:
Lugar: Washington, USA; Año: 1999 vol. 15 p. 500 - 500
ISSN:
8756-7938
Resumen:
A sulfonic group (up to 200 ìmol/mL membrane) was incorporated to epoxy-activated microporous hollow fibers to obtain high-capacity convective ion exchangers. The pure water flux through the membrane decreased exponentially with sulfonic group density and protein binding capacity incresed accordingly. At sulfonic group density of 70 ìmol/mL, the membrane lysozyme maximum binding capcity was 84 ± 9 mg/mL in comparison with its theorical monolayer maximum binding capacity of 20 mg/mL, thus evidencing tentacle formation. After a cycle of adsorption in a 30 mM sodium phosphate buffer, pH 7.0, adsorbed lysozyme could be quantitatively recovered following elution with 0.5 M NaCl in the same buffer. Dynamic capacity for lysozyme was 67% of maximum binding, and its value did not change at space velocities ranging from 10 to 40 min-1 as shown by the superimposition of the corresponding breakthrough curves. A cartridge assembled with 21 fibers showed a dynamic-to-static capacity ratio for lysozyme of 0.60 with 1 mg/mL pure lysozyme solution, and 0.42 with a particulate feed composed of 1 mg/mL lysozyme and 0.1 mg/mL yeast.