INVESTIGADORES
WOITOVICH VALETTI Nadia
artículos
Título:
Platform design for extraction and isolation of Bromelain: Complexformation and precipitation with carrageenan
Autor/es:
DÉBORA A. CAMPOS; NADIA WOITOVICH VALETTI; ANA OLIVEIRA; LORENZO M. PASTRANA-CASTRO; JOSÉ A. TEIXEIRA; MARIA M PINTADO; GUILLERMO PICÓ
Revista:
PROCESS BIOCHEMISTRY - (Print)
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2017 vol. 54 p. 156 - 161
ISSN:
1359-5113
Resumen:
The main objective of this work was to investigate for the first time the molecular mechanism of com-plex formation between bromelain (a positively charged enzyme) and carrageenan (a natural strongpolyelectrolyte, negatively charged) using spectroscopy techniques and thermodynamic approaches. TheBromelain-Carrageenan complex showed a maximal non-solubility at pH around 5.1. The solubility wasdependent on pH and ionic strength of the medium. To re-dissolve the formed complex, the pH waschanged and 500 mM of NaCl was added to the initial solution, proving the columbic mechanism forthe formation of non-soluble complex. The formation of the carrageenan-bromelain complex increasedin 8◦C the enzyme thermal stability, while its biological activity was not modified. The amount of totalenzyme recovered in solution after precipitation with around 0.08% w/v of carrageenan was 85?90%.