MATRICES DE POLIELECTROLITOS NATURALES: SU POTENCIAL APLICACIÓN EN LA BIOSEPARACIÓN DE ENZIMAS A NIVEL INDUSTRIAL

MARÍA EMILIA BRASSESCO; N. WOITOVICH; GUILLERMO PICÓ

Salamanca

Congreso; Congreso Iberoamericano de Biotecnología.; 2016

Sociedades española y portuguesas de biotecnologia

The equilibrium isotherms and adsorption kinetics of lysozyme on epichlorohydrin cross-linked alginate-guar gum matrix were studied. Kinetics adsorption followed a pseudo-first-order model while the isotherm equilibrium could be represented by the Freundlich equation. The maximal amount of lysozyme adsorbed onto this matrix was around 2.4 mg per g of hydrated matrix at pH 7.00. The adsorption mechanism was associated to a simple diffusion process with a weak columbic interaction between LZ and matrix. The presence of NaCl 0.3 M induced a total displacement of the LZ from the matrix. Under this condition, the percentage of desorbed protein was 95%. Successive cycles of adsorption-washing-elution were performed and the results demonstrate the reversibility of the process and the capacity of the method for enzyme purification and separation. A last successful step was carried out for the purification of LZ from white egg, like natural source. The model showed potential to be applied as a platform design in the isolation and purification of proteins.