Physicochemical characterization of a waste from argentine agriculture: Soybean Hull as potential tool for bio adsorption applied to the downstream processes of enzymes

CALORINE LE ROY; M EMILIA BRASSESCO; GUILLERMO PICÓ

Salamanca

Congreso; Congreso Iberoamericano de Biotecnología Bio Iberoamérica; 2016

Sociedad española y portuguesa de biotecnologia

Argentine produce 60 millions of tons of soybean/years, 7% represents the hull, a waste which is used as food supplement for cow or is discarded in the environment, producing a negative impact on it. In previous work we have recovered peroxidase from soy bean hull, however the non soluble fraction on this, is formed by polysaccharides with a great porosity which make a material with potential uses in adsorption process. The goal of this work was to evaluate the potential of soybean hull to be used as an absorbent matrix for macromolecules found in water waste. The matrix was chemically modified using four independent treatments: water washed (control), NaOH, citric and tartaric acids. The chemical structure modifications implied by the treatments (using Infra Red measurements), the number of carboxyl group present, the pH of electrical charge zero and the pKa were evaluated. Finally we evaluated the ability of soya hull to retain water and to absorb macromolecules.