DOWNSTREAM STRATEGY TO OBTAIN CHYMOTRYPSIN FROM BOVINE PANCREAS USING PRECIPITATION WITH AN ANIONIC STRONG POLYELECTROLYTE

BOERIS, V; ROMANINI, D; FARRUGGIA, B; PICÓ, G.

Recife Brasil

Workshop; I Workshop Internacional em Biotecnología; 2008

Universidad Federal de Pernambuco

A great amounts of enzymes is required for different industrial proposes which makes necessary the develop of scaling up methodologies. In the zone where is located our laboratory, the meat industries are very important therefore, great amounts of wastes from these are produced. In this work was analyzed a method for isolation and purification of chymotrypsin from bovine pancreas homogenate by formation of an insoluble complex between the enzyme and a synthetic strong negatively charge polyelectrolyte such as the sodium salt of poly vinyl sulfonate (PVS) [1]