INVESTIGADORES
PICO Guillermo Alfredo
artículos
Título:
Interaction behavior between chitosan and pepsin
Autor/es:
VALERIA BOERIS; YASMINE MICHELETTOB,; MARIA LIONZO; NÁDYA PESCE DA SILVEIRA; G. PICÓ
Revista:
CARBOHYDRATE POLYMERS
Editorial:
ELSEVIER SCI LTD
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 84 p. 459 - 464
ISSN:
0144-8617
Resumen:
The interaction between chitosan (a positively charged polysaccharide) and the acidic protein pepsin was studied by the use of dynamic light scattering, electronic spectroscopic approaches and measurements of thermodynamic functions. An interaction, mainly electrostatic, between the enzyme and the cationic polymer was found. The size of the soluble complexes formed between pepsin and chitosan was shown to depend on the pH value. Chitosan was seen to interact with the surface of the pepsin molecule, but no modification was observed in the pepsin secondary structure or in its chemical and thermal thermodynamic stability.
