INVESTIGADORES
PICO Guillermo Alfredo
artículos
Título:
The formation of non-soluble complexes between polyethyleneimineanions and their potential use to isolate enzymes
Autor/es:
CASSANE, C; BOERIS, V.; WAGNER, J.; BOERIS, V.; G, PICÓ
Revista:
COLLOIDS AND SURFACES B-BIOINTERFACES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2011 vol. 80 p. 354 - 358
ISSN:
0927-7765
Resumen:
The aqueous solution behavior of polyethyleneimine (a synthetic cationic polymer) in the presence of anions with two or more electrical charges (citrate, phosphate, sulphate, malate, malonate and succinate) was studied by means of turbidimetry and light scattering. Polyethyleneimine forms non-soluble complexes with these anions, which behave as a pseudo-polyampholyte with an isoelectrical pH value dependent on the type of anion. The effect of pH, polymer concentration and ionic strength on the nonsoluble complexes formation was examined. The complex precipitation pH range was between 3.5 and 8.0 and also depended on the type of anion. The complex formation was inhibited by the ionic strength in agreement with the electrostatic mechanism of the non-soluble complex formation. Model proteins with isoelectric pH from 1 to 10 were assayed in orden to be precipitated by these complexes. It was found that the non-soluble polyethyleneimineanion complexes have the property to precipitate macromolecules charged with an opposite electrical charge.