Precipitation of chymotrypsin from fresh bovine pancreas using i-carrageenan.

NADIA WOITOVICH VALETTI; J. LOMBARDI; V. BOERIS; G. PICÓ

PROCESS BIOCHEMISTRY

ELSEVIER

Lugar: Amsterdam; Año: 2012 vol. 47 p. 2570 - 2574

0032-9592

The separation of chymotrypsin from a crude filtrate of fresh bovine pancreas homogenate was carried  out using precipitation with a commercially available negatively charged natural strong polyelectrolyte:  -carrageenan. The zymogen form of the enzyme was activated by addition of trypsin (0.0001%, w/w),  then, the enzyme was precipitated by polyelectrolyte addition at pH 4.50. The non-soluble complex was  separated by simple centrifugation and re-dissolved by a pH change to 8.20. The recovery of chymotrypsin  biological activity was 60% of the initial activity in the homogenate with 3-fold increase in its specific activity. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up  to 10 times.-carrageenan. The zymogen form of the enzyme was activated by addition of trypsin (0.0001%, w/w),  then, the enzyme was precipitated by polyelectrolyte addition at pH 4.50. The non-soluble complex was  separated by simple centrifugation and re-dissolved by a pH change to 8.20. The recovery of chymotrypsin  biological activity was 60% of the initial activity in the homogenate with 3-fold increase in its specific activity. The volume of the final product decreased to 10% of the feedstock, concentrating the sample up  to 10 times.