INVESTIGADORES
WILLIAMS Federico Jose
artículos
Título:
Phosphate mediated adsorption and electron transfer of cytochrome c. A time-resolved SERR spectroelectrochemical study
Autor/es:
D. A. CAPDEVILA; W. A. MARMISOLLE; F. J. WILLIAMS; D. H. MURGIDA
Revista:
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
Editorial:
ROYAL SOC CHEMISTRY
Referencias:
Lugar: CAMBRIDGE; Año: 2013 vol. 15 p. 5386 - 5394
ISSN:
1463-9076
Resumen:
The study of proteins immobilized on biomimetic or biocompatible electrodes represents an active field
of research as it pursues both fundamental and technological interests. In this context, adsorption and
redox properties of cytochrome c (Cyt) on different electrode surfaces have been extensively reported,
although in some cases with contradictory results. Here we report a SERR spectroelectrochemical study
of the adsorption and electron transfer behaviour of the basic protein Cyt on electrodes coated with
amino-terminated monolayers. The obtained results show that inorganic phosphate (Pi) and ATP anions
are able to mediate high affinity binding of the protein with preservation of the native structure and
rendering an average orientation that guarantees efficient pathways for direct electron transfer. These
findings aid the design of Cyt-based bioelectronic devices and understanding the modulation by Pi and
ATP of physiological functions of Cyt.