INVESTIGADORES
MORENO Maria virginia
artículos
Título:
Endophytes from wheat as biocontrol agents against tan spot disease
Autor/es:
LARRAN S; SIMON MR; MORENO MV; SANTAMARINA S; PERELLÓ AE
Revista:
BIOLOGICAL CONTROL
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 92 p. 17 - 23
ISSN:
1049-9644
Resumen:
Endophytes from wheat cultivars isolated in Buenos Aires province, Argentina, were assessed for theirpotential as biocontrol agents against Pyrenophora tritici-repentis (Died.) Drechsler (anamorphDrechslera tritici-repentis) (Died.) Shoem (Dtr), the causal agent of tan spot of wheat. Endophytes werescreened using dual culture techniques and examining the effect on growth, sporulation and the antifungalactivity in greenhouse assays. The most of endophytes tested significantly reduced Dtr growth comparedto the control except Rhodotorula rubra from 11 to 15 days post inoculation. Trichoderma hamatum,Penicillium sp., Bacillus sp. and Paecilomyces lilacinus significantly reduced the colony diameter of thepathogen. Most of the endophytes evaluated showed morphological changes in the conidia and/or themycelia of D. tritici-repentis. In addition, two endophytes, Bacillus sp. and Fusarium sp., reduced signifi-cantly the percent spore germination of Dtr compared to the control by 82% and 52% respectively. Ingreenhouse experiment T. hamatum, Chaetomium globosum and Fusarium sp. significantly (P 6 0.05)reduced the average disease severity on all three leaves compared to the control. However, the bestantagonistic effect was shown with T. hamatum as it resulted in the greatest suppression in the greenhouseand in the dual-plate assays. Likewise, Bacillus sp. was other highlighted microorganism thatantagonized the pathogen in in vitro assays. From our promising results, we conclude that endophyteshave potential in the biological control of tan spot of wheat caused by D. tritici-repentis, particularlyT. hamatum and Bacillus sp.