Xanthophyll cycle activity and photosynthesis of Dunaliella tertiolecta (Chlorophyceae) and Thalassiosira weissflogii (Bacillariophyceae) during fluctuating solar radiation

VAN DE POLL, W.H; A.G.J. BUMA; R.J.W. VISSER; P.J. JANKNEGT; V.E. VILLAFAÑE; E.W. HELBLING

PHYCOLOGIA

INT PHYCOLOGICAL SOC

Año: 2010 vol. 49 p. 249 - 259

0031-8884

Short term UV radiation (280-400 nm) effects on xanthophyll cycle activity and photosynthesis were assessed during fluctuating irradiance (60 and 10 min cycles) for the marine algae Thalassiosira weissflogii (Bacillariophyceae) and Dunaliella tertiolecta (Chlorophyceae). Laboratory cultures were transported up and down the water column for 8 h under solar radiation, during which PSII quantum yield was monitored over 1 min intervals. In addition, pigment composition, xanthophyll de-epoxidation state and carbon assimilation were assessed during the fluctuating irradiance cycles. Although PSII quantum yield of both species mirrored irradiance, the PSII response to irradiance fluctuations changed over time as PSII quantum yield was down regulated at midday. This coincided with maximal xanthophyll de-epoxidation that developed during the course of the day for both species. In contrast to the de-epoxidation levels, non-photochemical quenching (NPQ) and PSII quantum yield fluctuated with the irradiance dynamics at noon in both species. Maximal xanthophyll de-epoxidation and NPQ at noon was lower under PAR+UV than under PAR exposure for T. weissflogii during the 10 min cycle, whereas this was not found for the 60 min cycle and in D. tertiolecta. Synthesis of xanthophyll cycle pigments occurred in both species, and was faster for D. tertiolecta during PAR+UV than during PAR exposure. Carbon incorporation and on most occasions PSII quantum yield were inhibited by UV exposure for both species, regardless of xanthophyll de-epoxidation state. UV effects on carbon assimilation were higher during 10 min than during 60 min irradiance fluctuation cycles. However, the 10 min irradiance fluctuation cycle appeared to enhance overall carbon assimilation in D. tertiolecta, but depressed productivity of T. weissflogii, as compared to the 60 min cycles.