Transmigration of different strains of Trypanosoma cruzi across a 3d-microtissue model.

RODRIGUEZ, ME; PERRONE, A; BUA, J; CAMPO, VA; LEVY, GV; SÁNCHEZ, DO; TEKIEL, V

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIB-SAP-SAIC-SAi, etc

TRANSMIGRATION OF DIFFERENT STRAINS OF TRYPANOSOMA CRUZI ACROSS A 3D-MICROTISSUE MODELThree-dimensional (3D) cultures are physiologically relevant because mimic the microarchitecture of tissues and provide an environment similar to the encountered in natural infections. Here, we studied the transmigration of T. cruzi trypomastigotes of different strains (DTUs I, II, V and VI) into 3D-microtissues (spheroids). Spheroids of HeLa cells expresing RFP (HeLa-RFP) were cultured with CFSE-labelled trypomastigotes; transmigration into spheroids was analyzed at 24 h post infection. 3D-reconstruction from confocal microscopy images showed a broad spectrum of migration among strains, being some strains much more migratory (>40 µm in depth) than others (